Application of proteomics in lab diagnosis
Sobhan Ghafourian1, Zamberi Sekawi, Mohammad Raftari
1Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran. sobhan.ghafurian@gmail.com
Clinical Laboratory
|July 20, 2013
Summary
This review explores proteomics, the large-scale study of proteins, and its diagnostic applications. It discusses proteomics methods, benefits, drawbacks, and its crucial role in understanding protein structure, function, and interactions.
Area of Science:
- Biochemistry
- Molecular Biology
- Clinical Diagnostics
Background:
- Proteomics involves the large-scale study of proteins, focusing on their structures and functions.
- Understanding protein roles is vital across various scientific disciplines.
Purpose of the Study:
- To introduce the applications of proteomics in laboratory diagnostics.
- To review the methodologies employed in proteomics.
- To discuss the advantages and disadvantages of proteomic approaches.
Main Methods:
- Review of established and emerging proteomics techniques.
- Analysis of data generated from proteomic studies.
- Comparative assessment of different proteomic platforms.
Main Results:
- Proteomics offers powerful tools for identifying and quantifying proteins.
- The review highlights the utility of proteomics in biomarker discovery and drug development.
- Challenges and limitations associated with current proteomic methods are discussed.
Conclusions:
- Proteomics is essential for elucidating protein structure, function, and interactions.
- Its application in lab diagnostics holds significant potential for advancing healthcare.
- Further development of proteomic technologies will enhance their clinical utility.
Related Concept Videos
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
01:19MALDI-TOF Mass Spectrometry
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
01:33Peptide Identification Using Tandem Mass Spectrometry
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...