Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Micelles01:30

Micelles

Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
Colloids03:22

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface Chemistry and Particle Morphology Govern the Multiscale Interactions and Properties of Silica-Polyelectrolyte-Stabilized Microcapsules.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

A study of in-the-column detector micrograph contrast in low-voltage scanning electron microscopy.

Micron (Oxford, England : 1993)·2025
Same author

A Nanostructural Study of Mixed Carbon and Boron Nitride Nanotube Solutions in Superacid using Cryogenic Electron Microscopy.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Non-canonical self-assembly of surfactant/cosurfactant mixtures detected by combining the power of SANS and cryo-TEM.

Journal of colloid and interface science·2025
Same author

Cryo-SEM in haematological research.

Journal of microscopy·2025
Same author

Lyotropic Liquid Crystalline Phase Behavior of Boron Nitride Nanotube Aqueous Dispersions.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: May 9, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
09:45

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Nanostructure formation in the lecithin/isooctane/water system.

Naama Koifman1, Maya Schnabel-Lubovsky, Yeshayahu Talmon

  • 1Department of Chemical Engineering and the Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa 32000, Israel.

The Journal of Physical Chemistry. B
|July 20, 2013
PubMed
Summary

This study reveals how lecithin concentration affects self-assembled nanostructures in oil-rich systems. Cryogenic electron microscopy visualized phase transitions from reverse micellar to lamellar and cubic phases.

More Related Videos

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes
09:47

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Published on: February 19, 2016

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
06:16

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

Published on: February 11, 2018

Related Experiment Videos

Last Updated: May 9, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
09:45

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes
09:47

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Published on: February 19, 2016

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
06:16

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

Published on: February 11, 2018

Area of Science:

  • Materials Science
  • Colloid and Surface Chemistry

Background:

  • Understanding self-assembled nanostructures is crucial for developing advanced materials.
  • Lecithin/isooctane/water systems are complex liquid mixtures with potential applications.

Purpose of the Study:

  • To characterize the self-assembled nanostructures in the lecithin/isooctane/water system using direct-imaging techniques.
  • To investigate the influence of lecithin concentration on nanostructural development and phase behavior.
  • To compare nanostructures formed by natural lecithin versus phosphatidylcholine.

Main Methods:

  • Cryogenic transmission electron microscopy (cryo-TEM) and cryogenic scanning electron microscopy (cryo-SEM) for direct imaging.
  • Small-angle X-ray scattering (SAXS) for structural confirmation.
  • Specimen preparation using a modified Controlled Environment Vitrification System (CEVS).

Main Results:

  • Identified nanostructural evolution with increasing lecithin concentration along the [water]/[lecithin] = 5 dilution line.
  • Observed phase transitions from reverse micellar to coexisting reverse micellar and lamellar phases, and finally to reverse liquid crystalline cubic and lamellar phases.
  • Demonstrated significant differences in phase behavior and nanostructure when using phosphatidylcholine compared to natural lecithin.

Conclusions:

  • Cryo-EM techniques are highly effective for nanostructural characterization of complex liquid systems.
  • The lecithin/isooctane/water system exhibits sensitivity to composition, requiring controlled specimen preparation.
  • Direct imaging of non-aqueous, concentrated complex liquids is feasible, expanding the utility of cryo-EM.