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Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
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Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
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Published on: June 20, 2018

Single nucleotide polymorphisms in caprine calpastatin gene.

R Sharma1, A Maitra, A K Pandey

  • 1National Bureau of Animal Genetic Resources, Karnal-132001, Haryana, India. rekvik@gmail.com

Genetika
|July 23, 2013
PubMed
Summary

Researchers identified novel genetic variations in the caprine calpastatin (CAST) gene, crucial for goat meat tenderness. This discovery aids in understanding and improving goat meat quality through genetic analysis.

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Area of Science:

  • Genetics
  • Animal Science
  • Molecular Biology

Background:

  • The calpain-calpastatin (CAST) system regulates meat tenderization.
  • CAST gene polymorphisms are linked to meat tenderness in cattle, sheep, and pigs.
  • Limited information exists on caprine CAST gene's impact on goat meat quality.

Purpose of the Study:

  • To investigate the polymorphic status of the caprine CAST gene.
  • To identify novel genetic variations within the caprine CAST gene.
  • To establish tools for further genetic polymorphism analysis in goats.

Main Methods:

  • Comparative sequencing of selected CAST gene regions (intron 5, 12, 3'UTR, exon 3, 7, 8, intron 7, 8).
  • Identification and characterization of single nucleotide polymorphisms (SNPs) and insertion/deletions (InDels).
  • Establishment of PCR-RFLP loci for genetic analysis.

Main Results:

  • Ten novel SNPs were identified in exon 3 and introns 5, 7, and 8.
  • A non-synonymous amino acid substitution (Lys/Arg) was detected in exon 3.
  • Twenty-four InDels were found in intronic regions and the 3'UTR.
  • Previously reported SNPs in intron 5, 3'UTR, and intron 12 were absent in this study.
  • High sequence homology was observed with bovine and ovine CAST sequences.

Conclusions:

  • Novel genetic variations in the caprine CAST gene were identified.
  • The study provides insights into genetic factors influencing goat meat quality.
  • Established PCR-RFLP loci facilitate future genetic polymorphism studies in indigenous goats.