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Updated: May 9, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
Detailed modulation of phenotypes and functions of bone marrow dendritic cells (BMDCs) by interferon-gamma (IFN-γ)
Ming Xue1, Linlin Zhu, Yiming Meng
1Department of Endodontics, China Medical University, Shenyang, People's Republic of China. alex_xm@sina.com
Abstract:
IFN-γ is a cytokine that plays crucial role in innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFN-γ is also a key activator of macrophages [1,2]. In the present study, we studied detailed modulation of IFN-γ on phenotypic and functional maturation of murine bone marrow derived dendritic cells (BMDCs). Phenotypic and functional maturation of BMDCs was evaluated by light microscope, flow cytometry(FCM), transmission electron microscopy (TEM), cytochemistry method, acid phosphatase activity(ACP), FITC-dextran bio-assay and enzyme linked immunosorbent assay (ELISA). We elucidated that IFN-γ up-regulated the expression of MHC II, CD40, CD80, CD83 and CD86 molecules on BMDCs, down-regulated the activity of pinocytosis and phagocytosis by BMDCs, and induced higher levels of IL-12 and TNF-α secreted by BMDCs. It is therefore confirmed that IFN-γ can effectively promote the maturation of BMDCs. Our study provides more evidence and rationale on future application of IFN-γ for enhancing host immunity.
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