[Changes in the superoxide dismutase activity during stimulation of polymorphonuclear leukocytes of peripheral blood]

The functional activity of the peripheral blood polymorphonuclear leukocytes (PML) was investigated by using the method of latex-stimulated luminol-dependent chemiluminescence (CL). The CL-intensity of PML taken from patients with acute myocardial infarction (MI) was found to be 20 times higher than that of normal individuals (NI). The change in activity of endogenous antioxidative enzyme systems may account for alteration of PML CL-parameters. It was established that the initial superoxide dismutase (SOD) activity of unstimulated PML from patients with MI exceeds that of NI, and that rapid increase in intercellular SOD activity (within 30 sec.) occurs in the process of PML stimulation. It was suggested that the change of SOD activity during PML stimulation was the result in the enzyme partial proteolysis in the cells. The positive correlation between initial level of SOD activity and CL-intensity of PML was observed. The investigation of the above parameters in MI dynamic showed a gradual normalization of PML CL-response and insignificant decrease in intracellular SOD activity in case of a favourable cause of the disease. Increased SOD activity in PMLs may be one of the factors contributing to a decrease in PML functional activity in the disease dynamic.