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Related Experiment Video

Updated: May 9, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

Two-photon voltage imaging using a genetically encoded voltage indicator.

Walther Akemann1, Mari Sasaki, Hiroki Mutoh

  • 1RIKEN Brain Science Institute, Hirosawa, Wako-City, 351-0198 Saitama, Japan.

Scientific Reports
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

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Two-photon imaging of voltage-sensitive fluorescent proteins (VSFPs) enables cellular-resolution voltage recording in mouse cortex. This genetically-encoded voltage indicator (GEVI) approach overcomes limitations of previous methods for in-vivo neural activity monitoring.

Area of Science:

  • Neuroscience
  • Biophysics
  • Optical Imaging

Background:

  • Voltage-sensitive fluorescent proteins (VSFPs) are genetically-encoded voltage indicators (GEVIs) used for monitoring neural activity.
  • Previous in-vivo imaging with 1-photon (1P) microscopy was limited to superficial layers and lacked single-neuron resolution.
  • Two-photon (2P) excitation microscopy has not been widely applied to GEVI experiments.

Purpose of the Study:

  • To investigate the feasibility and performance of 2P imaging with VSFPs in the mouse cortex.
  • To compare 2P imaging of VSFPs with 1P imaging.
  • To achieve cellular-resolution voltage imaging in vivo.

Main Methods:

  • Utilized VSFP Butterfly 1.2 expressed in pyramidal neurons.
  • Performed 2P fluorescence excitation imaging in acute brain slices and in vivo in anesthetized mice.

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Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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Published on: December 22, 2020

Related Experiment Videos

Last Updated: May 9, 2026

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
09:57

Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

Published on: February 4, 2016

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08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

  • Recorded sensory responses to whisker deflection in the somatosensory cortex.

    • Main Results:

    • 2P imaging of VSFP Butterfly 1.2 in brain slices showed voltage changes consistent with 1P imaging, but with a 2-3 fold larger ΔR/R signal.
    • In vivo 2P imaging achieved cellular resolution in layer 2/3 of the mouse cortex.
    • Sensory-evoked voltage responses were recorded at full frame video rate in the somatosensory cortex.

    Conclusions:

    • 2P imaging is a feasible and effective method for GEVI-based voltage recording in the mouse cortex.
    • This technique offers improved signal-to-noise and cellular resolution compared to 1P imaging.
    • GEVI-based 2P imaging opens new possibilities for studying neural circuit function at the cellular level in vivo.