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Related Concept Videos

Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...
Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Histology of the Small Intestine01:27

Histology of the Small Intestine

The small intestine exhibits a unique histological structure that significantly enhances its function in digestion and nutrient absorption. These structures include circular folds, villi, and various specialized cells that collectively facilitate the digestion of food.
The intestinal lining features transverse folds called circular folds, each housing fingerlike projections known as intestinal villi. These villi are covered by a layer of simple columnar epithelium, also referred to as...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

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Related Experiment Video

Updated: May 9, 2026

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
07:48

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

The intestinal crypt, a prototype stem cell compartment.

Hans Clevers1

  • 1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, 3584 CT Utrecht, the Netherlands. h.clevers@hubrecht.eu

Cell
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

The intestinal epithelium serves as a key model for adult stem cell research. Studies using transgenic mice reveal novel self-renewal processes in intestinal stem cells that challenge traditional stem cell definitions.

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Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
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Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

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Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models
07:48

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Published on: November 21, 2015

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

Area of Science:

  • Stem cell biology
  • Gastrointestinal research
  • Regenerative medicine

Background:

  • The intestinal epithelium is a dynamic tissue with rapid self-renewal.
  • Adult stem cells in the gut are crucial for tissue homeostasis and repair.
  • Understanding intestinal stem cell behavior is vital for treating gastrointestinal diseases.

Purpose of the Study:

  • To investigate the self-renewal kinetics and architecture of intestinal stem cells.
  • To utilize advanced mouse models for in vivo stem cell tracking.
  • To explore novel stem cell behaviors that deviate from classical definitions.

Main Methods:

  • Employing transgenic mouse models for in vivo visualization.
  • Utilizing genetic lineage tracing to track stem cell progeny.
  • Isolating fluorescently marked stem cells for molecular analysis.
  • Culturing stem cells in vitro to generate "mini-guts".

Main Results:

  • Demonstrated intense self-renewal kinetics in intestinal stem cells.
  • Visualized and traced individual stem cells and their offspring in vivo.
  • Successfully cultured intestinal stem cells to create self-renewing "mini-guts" in vitro.
  • Identified stem cell behaviors that challenge classical stem cell paradigms.

Conclusions:

  • The intestinal epithelium provides a powerful model for studying adult stem cell dynamics.
  • Transgenic mouse models offer unprecedented insights into stem cell behavior.
  • Intestinal stem cell self-renewal exhibits unique characteristics, including defiance of classical stem cell definitions.