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Updated: May 9, 2026

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Quantitative analysis of three-dimensional fluorescence localization microscopy data.

Dylan M Owen¹, David J Williamson, Lies Boelen

¹Centre for Vascular Research and Australian Centre for Nanomedicine, University of New South Wales, Sydney, Australia.

Biophysical Journal

|July 23, 2013

Summary

This summary is machine-generated.

We developed a novel 3D imaging analysis method to precisely map subcellular structures and protein distribution, even within complex cell membranes. This technique overcomes previous limitations in visualizing nanoscale cellular organization.

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Last Updated: May 9, 2026

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Cell Biology
Biophysics
Microscopy

Background:

Determining the 3D molecular organization of subcellular organelles in intact cells is difficult.
Existing methods struggle to resolve structures below the diffraction limit.

Purpose of the Study:

To present a new analysis approach for 3D localization microscopy.
To identify and quantify subcellular objects, their shape, and volume.

Main Methods:

Utilizing 3D localization microscopy.
Developing a novel analysis approach for high-resolution imaging.
Applying the method to map plasma membrane topography.

Main Results:

Successfully identified subcellular objects below the diffraction limit.
Quantified the shape and volume of these organelles.
Mapped the topography of the plasma membrane and protein distribution.

Conclusions:

The new approach enables detailed 3D analysis of subcellular structures.
It is particularly effective for studying complex membrane architectures and protein localization.