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Related Experiment Video

Updated: May 9, 2026

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
13:12

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

Published on: April 4, 2011

Greasing the SCIDs for universal flu antibodies.

Jonathan W Yewdell1, William L Ince

  • 1Laboratory of Viral Diseases, NIAID, Bethesda, MD 20892, USA. jyewdell@nih.gov

Cell Host & Microbe
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a SCID mouse method to isolate human monoclonal antibodies (mAbs) from B cells. This technique successfully identified influenza A virus-specific antibodies for potential therapeutic use.

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Last Updated: May 9, 2026

Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
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A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
04:47

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

Area of Science:

  • Immunology
  • Virology
  • Biotechnology

Background:

  • Developing effective antibody-based therapies requires efficient methods for isolating human monoclonal antibodies (mAbs).
  • Existing methods may face challenges in specificity and scalability for therapeutic applications.

Purpose of the Study:

  • To present a robust SCID mouse-based method for isolating human mAbs with specificities of interest.
  • To demonstrate the utility of this method by isolating human mAbs against influenza A virus.

Main Methods:

  • Adoptive transfer of human B cells into severe combined immunodeficient (SCID) mice.
  • Isolation and characterization of human monoclonal antibodies (mAbs) from B cells within the SCID mouse model.
  • Testing the specificity and potential efficacy of isolated mAbs against influenza A virus strains.

Main Results:

  • A reliable method for isolating human mAbs from SCID mice was established.
  • Human mAbs with potential neutralizing activity against influenza A virus were successfully isolated.
  • The isolated mAbs demonstrated specificity for targeting influenza A virus.

Conclusions:

  • The described SCID mouse model provides a powerful platform for isolating human monoclonal antibodies (mAbs) of desired specificity.
  • This method holds promise for the development of novel antibody-based therapeutics, particularly for infectious diseases like influenza A virus.
  • Further development could lead to broadly protective influenza antibodies.