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Related Experiment Video

Updated: May 9, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Lysozyme detection on aptamer functionalized graphene-coated SPR interfaces.

Palaniappan Subramanian1, Adam Lesniewski, Izabela Kaminska

  • 1Institut de Recherche Interdisciplinaire (IRI), CNRS USR 3078, Université Lille1, Parc de la Haute Borne, 50 avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.

Biosensors & Bioelectronics
|July 23, 2013
PubMed
Summary

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This study presents a graphene-based surface plasmon resonance (SPR) biosensor for detecting lysozyme. The novel sensor achieves sensitive and selective protein detection with a low limit of 0.5 nM.

Area of Science:

  • Biosensing
  • Nanotechnology
  • Surface Chemistry

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical technique for detecting molecular interactions.
  • Graphene and its derivatives offer unique electronic and surface properties for biosensor development.
  • Lysozyme is a model protein frequently used in biosensing studies.

Purpose of the Study:

  • To develop a sensitive and selective SPR-based biosensor for lysozyme detection.
  • To utilize reduced graphene oxide (rGO) functionalized with DNA aptamers for enhanced biosensing.
  • To demonstrate a label-free detection method for proteins.

Main Methods:

  • Fabrication of an SPR sensor chip using a gold film coated with rGO via electrophoretic deposition of graphene oxide (GO).
Keywords:
AptamerElectrophoretic depositionLysozymeReduced graphene oxideSurface plasmon resonance

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Last Updated: May 9, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

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  • Functionalization of the rGO surface with anti-lysozyme DNA aptamers through π-stacking interactions.
  • Detection of lysozyme using the functionalized SPR biosensor.

    • Main Results:

    • The rGO film thickness was controlled by deposition time, with optimal results for 20 s pulses.
    • The graphene-based SPR biosensor demonstrated selective detection of lysozyme.
    • A label-free, concentration-dependent detection of lysozyme was achieved with a detection limit of 0.5 nM.

    Conclusions:

    • The developed rGO-functionalized SPR biosensor is effective for sensitive and selective lysozyme detection.
    • Electrophoretic deposition provides a controllable method for preparing rGO films for SPR applications.
    • This biosensor platform shows promise for label-free protein analysis.