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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Graphene-based nanoprobes and a prototype optical biosensing platform.

Hui Xu1, Dongfang Wang, Shijiang He

  • 1School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.

Biosensors & Bioelectronics
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

We created a graphene oxide nanoprobe using anti-immunoglobulin G and horseradish peroxidase for enhanced biomolecule detection. This new probe offers improved sensitivity for molecular diagnostics, surpassing traditional methods like ELISA.

Keywords:
Cancer markerGraphene oxide (GO)ImmunoassayNanoprobeSignal amplification

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Last Updated: May 9, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
07:51

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

Published on: February 1, 2022

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
09:39

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Published on: March 31, 2022

Area of Science:

  • Nanomaterials Science
  • Biochemistry
  • Biomedical Engineering

Background:

  • Graphene's biomedical applications depend on biomolecule interactions.
  • Developing sensitive diagnostic tools is crucial for early disease detection.

Purpose of the Study:

  • To develop a graphene oxide-based signal-amplification nanoprobe.
  • To investigate the structure and function of horseradish peroxidase (HRP) on graphene oxide (GO).
  • To assess the nanoprobe's efficacy in a sandwich immunoassay for cancer marker detection.

Main Methods:

  • Co-adsorption of anti-immunoglobulin G (anti-IgG) and HRP onto graphene oxide (GO).
  • Investigation of HRP activity and structure on the GO nano-interface.
  • Application of the bifunctional nanoprobes in a sandwich-type immunoassay.

Main Results:

  • HRP retained significant native activity (78%) and α-helix content (77%) on the GO interface.
  • The bifunctional nanoprobes demonstrated improved binding and signal amplification.
  • Achieved a detection limit of 10 pg/mL for alpha-fetoprotein (AFP), outperforming conventional ELISA.

Conclusions:

  • Co-adsorption preserves biomolecule bioactivity, avoiding chemical conjugation issues.
  • The easily fabricated GO-based nanoprobes offer enhanced sensitivity for molecular diagnostics.
  • These nanoprobes show potential as universal probes for various diagnostic applications.