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Updated: May 9, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
Development of buffers for fast semidry transfer of proteins
Dušan Garić1, Laure Humbert, Nadège Fils-Aimé
1Division of Hematology, Department of Medicine, Royal Victoria Hospital, McGill University Health Centre, McGill University, Montreal, QC H3A 1A1, Canada.
Abstract:
Western blot is an extensively used method for protein detection in cell biology. To optimize this procedure, here we examined a panel of buffers for their ability to efficiently transfer proteins from SDS-polyacrylamide gels onto nitrocellulose membranes in a short 12-min period, designated here as fast semidry transfer. Our results show for the first time that HEPES- and HEPPS/EPPS-based buffers represent the most efficient buffers for fast semidry transfer.

