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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
Published on: May 20, 2022
A new correlation based alignment technique for use in electron tomography
1NanoSciences Innovation Centre, Department of Physics, University of Cape Town, Rondebosch 7701, South Africa.
Abstract:
In this paper we present a new correlation based method for the alignment of a single axis tilt series. Rather than performing the pairwise correlation procedure with the central image as the starting point, the method presented here calculates the optimal starting position within the tilt series and proceeds towards both ends. The starting position is determined by maximisation of a viability function, J, which rewards cumulative series correlation and penalises both cumulative series shift and distance from the centre of the image series.
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