Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Karyotyping01:17

Karyotyping

Overview
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Communication-efficient decentralized clustering for dynamical multi-agent systems.

PloS one·2025
Same author

Multilevel modeling and control of dynamic systems.

Scientific reports·2024
Same author

Comparative Bioinformatic Analysis Reveals Conserved Regions in SARS-CoV-2 Genome for RAPID Pandemic Response.

International journal of molecular sciences·2024
Same author

Indoor Navigation in Facilities with Repetitive Structures.

Sensors (Basel, Switzerland)·2024
Same author

Electroencephalography functional connectivity-A biomarker for painful polyneuropathy.

European journal of neurology·2022
Same author

Entropy-Based Approach for the Detection of Changes in Arabic Newspapers' Content.

Entropy (Basel, Switzerland)·2020
Same journal

TBX6 promotes proliferation, invasion, and migration in colorectal cancer: Integrated transcriptomic and protein interaction network analysis.

Gene·2026
Same journal

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
See all related articles

Related Experiment Video

Updated: May 9, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

Hidden ancient repeats in DNA: mapping and quantification.

Zakharia M Frenkel1, Zeev Barzily, Zeev Volkovich

  • 1Department of Software Engineering, ORT Braude College, Karmiel, Israel. zakharf@research.haifa.ac.il

Gene
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

Most genomic sequences evolved from tandem repeat expansions, particularly triplet repeats. An algorithm reveals that approximately 35.5% of the E. coli K12 genome originated from simple repeats, confirming their crucial role in gene evolution.

Keywords:
ASCIIAmerican Standard Code for Information InterchangeAncient repeatsCDSsE. coliEscherichia coliMsS. cerevisiaeSaccharomyces cerevisiaeText miningTriplet expansioncoding sequencesminimal fragment size

More Related Videos

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Related Experiment Videos

Last Updated: May 9, 2026

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Area of Science:

  • Genomics
  • Molecular Evolution
  • Bioinformatics

Background:

  • Tandem repeating sequences, especially triplet repeats, are crucial in gene evolution.
  • Genomic sequences may have evolved through repeat expansions and subsequent changes.

Purpose of the Study:

  • To estimate the proportion of genomic sequences with a repeat origin.
  • To adapt a dynamic programming algorithm for mapping ancient repeat expansion events.

Main Methods:

  • Adaptation of a text segmentation algorithm based on dynamic programming.
  • Maximizing segmentation cost by assessing similarity to putative repeat sequences.
  • Analysis of genomic sequences and their shuffled counterparts.

Main Results:

  • Natural genomic sequences show longer fragments and higher similarity to repeats than shuffled sequences.
  • Coding sequences permit repeats only when word size is a multiple of three.
  • Non-coding sequences accommodate repeats of all word sizes.
  • Approximately 35.5% of the Escherichia coli K12 genome is traceable to simple repeat ancestors.

Conclusions:

  • The findings strongly support the hypothesis of triplet expansions being central to gene origin and evolution.
  • The study sheds light on genomic sequence organization and the evolutionary significance of repeats.