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Updated: May 9, 2026
08:56
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Aptasensor and genosensor methods for detection of microbes in real world samples
1UPMC Univ Paris 06, UMR 7621, LOMIC, Observatoire Océanologique, F-66650 Banyuls/mer, France; CNRS, UMR 7621, LOMIC, Observatoire Océanologique, F-66650 Banyuls/mer, France.
Methods (San Diego, Calif.)
|July 23, 2013
Summary
Rapid detection of microorganisms is crucial for public health. This review explores advanced DNA-based biosensors, genosensors and aptasensors, offering sensitive and specific alternatives to traditional methods.
Area of Science:
- Biotechnology
- Biosensor Technology
- Molecular Diagnostics
Background:
- Traditional microbiological methods are time-consuming, labor-intensive, and not suitable for rapid, on-site analysis.
- Growing concerns regarding food and environmental safety necessitate advanced detection techniques for microorganisms.
- Nucleic acid-based biosensors offer promising solutions due to their specificity and sensitivity.
Purpose of the Study:
- To review recent significant advances in DNA-based biosensors, specifically genosensors and aptasensors.
- To highlight innovative methods and trends in the development of these biosensors.
- To provide an overview of biorecognition element selection, immobilization strategies, and sensing formats.
Main Methods:
- Review of recent scientific literature focusing on genosensors and aptasensors.
- Analysis of DNA/RNA hybridization principles in genosensors.
- Examination of aptamer-based recognition mechanisms in aptasensors.
Main Results:
- Genosensors detect targets via DNA/RNA hybridization with single-stranded DNA (ssDNA) probes.
- Aptasensors utilize aptamers, which bind targets with high affinity and specificity, as bioreceptors.
- Recent trends include novel biorecognition element selection, advanced DNA immobilization techniques, and diverse sensing formats.
Conclusions:
- Genosensors and aptasensors represent significant advancements in microorganism detection.
- These DNA-based biosensors offer rapid, specific, and sensitive alternatives to conventional methods.
- Continued innovation in selection, immobilization, and sensing formats will further enhance biosensor capabilities for public health applications.
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