Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Olaparib-mediated enhancement of 5-fluorouracil cytotoxicity in mismatch repair deficient colorectal cancer cells.

BMC cancer·2026
Same author

Higher Levels of <i>BRCA1</i> Gene Methylation in Sporadic Breast Cancer Patients with a Lower Incidence of Recurrence.

Medical sciences (Basel, Switzerland)·2026
Same author

Pharmacological Study of Imidazolium and Pyridinium Salts with Antifungal Activity against Chromoblastomycosis Agents.

Current topics in medicinal chemistry·2026
Same author

Morphofunctional Heterogeneity and Plasticity of Glioblastoma Cells Induced to Senescence by Temozolomide.

Aging cell·2026
Same author

Colorectal cancer cells respond differentially to autophagy induced by tyrosine kinase inhibitors.

Scientific reports·2026
Same author

Exploratory biomarkers for oxaliplatin-induced nivolumab responsiveness in metastatic microsatellite-stable colorectal cancer.

British journal of cancer·2026

Related Experiment Video

Updated: May 9, 2026

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites
09:39

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites

Published on: August 2, 2024

DNA alkylation damage and autophagy induction.

Diana L Bordin1, Michelle Lima1, Guido Lenz1

  • 1Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500, CEP 91501-970 Porto Alegre, RS, Brazil.

Mutation Research
|July 23, 2013
PubMed
Summary

Alkylating agents cause DNA damage, triggering cellular responses including autophagy. This review explores how autophagy impacts chemotherapy effectiveness, potentially causing chemoresistance or cell death.

Keywords:
Alkylating agentsAutophagyDNA damage

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Experiment Videos

Last Updated: May 9, 2026

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites
09:39

Proximity Ligand Assay to Localize Proteins in DNA Damage Sites

Published on: August 2, 2024

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Area of Science:

  • Oncology
  • Molecular Biology
  • Cellular Biology

Background:

  • Alkylating agents are widely used chemotherapy drugs that induce DNA damage.
  • Cancer cells exhibit complex responses to DNA damage, including cell cycle arrest and DNA repair.
  • Autophagy induction is a recently observed response to DNA-targeting drugs like alkylating agents.

Purpose of the Study:

  • To review the primary alkylating agents used in clinical oncology.
  • To elucidate the cellular responses elicited by these agents, with a specific focus on autophagy.
  • To clarify the dual role of autophagy in cancer cell response to chemotherapy.

Main Methods:

  • Literature review of clinical oncology studies.
  • Analysis of cellular signaling cascades following DNA damage.
  • Examination of research on autophagy induction by alkylating agents.

Main Results:

  • Alkylating agents cause diverse DNA damage, initiating signaling pathways for survival and repair.
  • Autophagy induction in cancer cells treated with alkylating agents can delay apoptosis.
  • The role of autophagy in chemotherapy is context-dependent, potentially leading to chemoresistance or cell death.

Conclusions:

  • Autophagy's role in chemotherapy response is complex and debated.
  • Understanding autophagy is crucial for overcoming chemoresistance.
  • Further research is needed to define autophagy's precise impact on chemotherapy outcomes.