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Updated: May 9, 2026

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Published on: June 20, 2019
Clostridium acidurici electron-bifurcating formate dehydrogenase
Shuning Wang1, Haiyan Huang, Jörg Kahnt
1Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Abstract:
Cell extracts of uric acid-grown Clostridium acidurici catalyzed the coupled reduction of NAD(+) and ferredoxin with formate at a specific activity of 1.3 U/mg. The enzyme complex catalyzing the electron-bifurcating reaction was purified 130-fold and found to be composed of four subunits encoded by the gene cluster hylCBA-fdhF2.
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