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Related Experiment Video

Updated: May 9, 2026

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
10:13

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Published on: August 23, 2015

Vitrigel-eye irritancy test method using HCE-T cells.

Hiroyuki Yamaguchi1, Hajime Kojima, Toshiaki Takezawa

  • 1* Division of Animal Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan;

Toxicological Sciences : an Official Journal of the Society of Toxicology
|July 23, 2013
PubMed
Summary
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A new Vitrigel-EIT method accurately predicts chemical eye irritancy using a human corneal epithelium model. This test shows high accuracy and sensitivity, identifying potential irritants without false negatives.

Area of Science:

  • Ophthalmology
  • Toxicology
  • Biomedical Engineering

Background:

  • Previous research correlated transepithelial electrical resistance (TEER) changes with ocular irritancy.
  • A novel collagen vitrigel membrane (CVM) chamber was developed for advanced 3D cell culture and biomedical assays.

Purpose of the Study:

  • To develop and validate a new eye irritancy test (EIT) method, termed Vitrigel-EIT, for rapid and accurate chemical irritancy assessment.
  • To apply the TEER assay system to a human corneal epithelium (HCE) model within the CVM chamber.

Main Methods:

  • A Vitrigel-HCE model was created by culturing HCE-T cells in the CVM chamber for 6 days, forming a six-layer epithelium with in vivo characteristics.
  • TEER values were measured over time after exposing the model to 30 chemicals.
Keywords:
HCE-T cells.collagen vitrigel membranecorneal epitheliumeye irritation testtransepithelial electrical resistance

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Related Experiment Videos

Last Updated: May 9, 2026

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
10:13

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model

Published on: August 23, 2015

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells
09:31

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Published on: July 22, 2021

  • Data were analyzed using three indexes (time lag, intensity, plateau level) for classification.

    • Main Results:

    • The Vitrigel-EIT method successfully classified chemicals into irritant and non-irritant categories with 100% sensitivity, 75% specificity, and 90% accuracy, correlating well with GHS classification.
    • The method demonstrated the ability to predict widespread eye irritancy without false negatives.
    • Immunohistology revealed tight junction disruption in some GHS-classified non-irritants, suggesting potential mild irritancy.

    Conclusions:

    • The Vitrigel-EIT method offers a reliable and sensitive approach for predicting chemical eye irritancy.
    • This novel method enhances safety assessment by identifying potential irritants, including those with mild properties not captured by current classifications.