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Updated: May 9, 2026
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ROS Live Cell Imaging During Neuronal Development
Published on: February 9, 2021
Imaging ROS signaling in cells and animals
Xianhua Wang1, Huaqiang Fang, Zhanglong Huang
1State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, Beijing Key Laboratory of Cardiometabolic Molecular Medicine, Peking University, Beijing 100871, China.
Journal of Molecular Medicine (Berlin, Germany)
|July 23, 2013
Summary
Reactive oxygen species (ROS) are vital cellular messengers implicated in diseases. Advanced ROS imaging techniques now allow for high-resolution visualization, revealing new insights into cellular signaling and disease mechanisms.
Area of Science:
- Biochemistry
- Cell Biology
- Medical Imaging
Background:
- Reactive oxygen species (ROS) play dual roles as signaling molecules and oxidative stressors.
- Dysregulation of ROS is linked to major pathologies including cancer, cardiovascular, and neurodegenerative diseases.
- Accurate visualization and quantification of ROS dynamics are crucial for understanding their complex roles.
Purpose of the Study:
- To provide an updated review of current reactive oxygen species (ROS) imaging technologies.
- To highlight recent advancements in ROS detection and visualization.
- To identify knowledge gaps and emerging research directions in ROS imaging.
Main Methods:
- Development of novel synthetic and genetically encoded fluorescent ROS indicators.
- Utilization of protein indicator-expressing animal models for in vivo studies.
- Advancements in in vivo imaging technologies for ROS detection.
Main Results:
- Significant progress in ROS imaging across cellular, tissue, and organismal levels.
- Discovery of mitochondrial superoxide flashes as key signaling events.
- Identification of hydrogen peroxide transients involved in wound healing.
Conclusions:
- Innovative ROS imaging has led to significant discoveries in cellular signaling.
- Further research is needed to address current limitations in ROS detection.
- Emerging directions promise deeper understanding of ROS in health and disease.
