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DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
Published on: September 28, 2021
Acquisition of thin coronal sectional dataset of cadaveric liver
Li Lou1, Shu Wei Liu, Zhen Mei Zhao
1Research Center for Sectional and Imaging Anatomy, School of Medicine, Shandong University, 44#, Wenhua Xi Road, Jinan, 250012, Shandong, People's Republic of China.
Purpose:
To obtain the thin coronal sectional anatomic dataset of the liver by using digital freezing milling technique.
Methods:
The upper abdomen of one Chinese adult cadaver was selected as the specimen. After CT and MRI examinations verification of absent liver lesions, the specimen was embedded with gelatin in stand erect position and frozen under profound hypothermia, and the specimen was then serially sectioned from anterior to posterior layer by layer with digital milling machine in the freezing chamber. The sequential images were captured by means of a digital camera and the dataset was imported to imaging workstation.
Results:
The thin serial section of the liver added up to 699 layers with each layer being 0.2 mm in thickness. The shape, location, structure, intrahepatic vessels and adjacent structures of the liver was displayed clearly on each layer of the coronal sectional slice. CT and MR images through the body were obtained at 1.0 and 3.0 mm intervals, respectively.
Conclusion:
The methodology reported here is an adaptation of the milling methods previously described, which is a new data acquisition method for sectional anatomy. The thin coronal sectional anatomic dataset of the liver obtained by this technique is of high precision and good quality.
