Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...

Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-Potential Coulometry: Electrolytic Methods

Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential ensures...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Risk assessment of the mechanical spread of bacterial pathogens due to Lasius neglectus ants infesting a tertiary hospital.

The Journal of hospital infection·2024

Same author

Abnormal thyroid function is common in takotsubo syndrome and depends on two distinct mechanisms: results of a multicentre observational study.

Journal of internal medicine·2020

Same author

Systemic inflammatory response after endoscopic surgery of Zenker's diverticulum.

Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale·2019

Same author

Calcium channel gating.

Pflugers Archiv : European journal of physiology·2018

Same author

Correlation between human ether-a-go-go-related gene channel inhibition and action potential prolongation.

British journal of pharmacology·2017

Same author

Analysis of β-Subunit-dependent GABAA Receptor Modulation and Behavioral Effects of Valerenic Acid Derivatives.

The Journal of pharmacology and experimental therapeutics·2016

Search research articles

Related Experiment Video

Updated: May 9, 2026

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

Published on: January 20, 2019

Methods for quantification of pore-voltage sensor interaction in Ca(V)1.2.

S Beyl¹, P Kügler, A Hohaus

¹Department of Neurophysiology and Neuropharmacology, Medical University of Vienna, Schwarzspanierstrasse 17, 1090, Vienna, Austria.

Pflugers Archiv : European Journal of Physiology

|July 23, 2013

Summary

We developed a new method to analyze voltage-gated ion channel kinetics. This technique quantifies voltage sensor movements and pore transitions, offering insights into channel gating mechanisms.

More Related Videos

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Published on: March 23, 2015

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

Related Experiment Videos

Last Updated: May 9, 2026

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium

Published on: January 20, 2019

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer

Published on: March 23, 2015

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

Area of Science:

Biophysics
Molecular Biology
Ion Channel Physiology

Background:

Voltage sensors (VSs) control the opening and closing of voltage-gated ion channels.
Understanding VS movement and pore transitions is crucial for ion channel function.

Purpose of the Study:

To propose a novel technique for estimating equilibrium and rate constants of voltage sensor movements and pore transitions.
To analyze CaV1.2 channel kinetics using a four-state model.

Main Methods:

Analysis of macroscopic current kinetics.
Modeling of bell-shaped voltage dependence of activation/deactivation time constants.
Application of Boltzmann distributions for CaV1.2 activation analysis.
Utilizing a circular four-state channel model (rest, activated, open, deactivated).

Main Results:

The proposed technique uniquely constrains model parameters for CaV1.2.
Pore mutations on IS6-IVS6 segments significantly shift the VS equilibrium.
A channelopathy mutation (I781T) on IIS6 primarily shifts VS equilibrium (65%) rather than destabilizing the pore (35%).

Conclusions:

The developed algorithm accurately estimates rate and equilibrium constants from macroscopic currents.
This method provides a deeper understanding of voltage sensor gating and its role in channelopathies.
The technique is potentially applicable to other voltage-gated ion channels.