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Updated: May 9, 2026

Targeted Laser Ablation in the Embryo of Saccharina latissima

Targeted Laser Ablation in the Embryo of Saccharina latissima

Published on: March 11, 2022

Laser-mediated cell ablation during post-implantation mouse development.

Jesse R Angelo¹, Kimberly D Tremblay

¹Department of Veterinary and Animal Science, University of Massachusetts, Amherst, Massachusetts.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|July 23, 2013

Summary

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Laser ablation precisely targets cells in mouse embryos, enabling new studies of early development and organ formation. This technique is compatible with normal embryonic development and aids in understanding cell fate.

Area of Science:

Developmental Biology
Mammalian Embryogenesis
Cell Biology

Background:

Laser-mediated cell ablation is a valuable tool for studying cell fate in externally developing organisms.
Its application in mammalian post-implantation development has been limited.

Purpose of the Study:

To develop and validate a laser ablation technique for precise cell targeting in mouse embryos.
To assess the compatibility of laser ablation with mammalian post-implantation development.
To investigate the role of specific progenitor populations in organogenesis.

Main Methods:

Pairing laser ablation with murine embryo culture.
Establishing precise ablation parameters with minimal disruption to adjacent cells.
Ablating endoderm and specific liver bud progenitor domains.

Keywords:

embryo culture endoderm laser ablation liver development mouse

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Last Updated: May 9, 2026

Targeted Laser Ablation in the Embryo of Saccharina latissima

Targeted Laser Ablation in the Embryo of Saccharina latissima

Published on: March 11, 2022

Deep and Spatially Controlled Volume Ablations using a Two-Photon Microscope in the Zebrafish Gastrula

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Published on: July 15, 2021

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

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Main Results:

Laser ablation was precisely performed with minimal disruption.
Ablation of a large endoderm domain resulted in a phenotypically normal embryo and gut tube after culture.
Targeted ablation of a single liver bud progenitor domain caused a unilateral delay in liver bud formation.

Conclusions:

Laser ablation is a specific and effective technique for studying cell fate in mouse embryos.
This method advances developmental studies in mice.
It provides insights into organ specification, differentiation, cell migration, and tissue interactions.