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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...

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Related Experiment Video

Updated: May 9, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

A TAF4 coactivator function for E proteins that involves enhanced TFIID binding.

Wei-Yi Chen1, Jinsong Zhang, Huimin Geng

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10065, USA.

Genes & Development
|July 23, 2013
PubMed
Summary
This summary is machine-generated.

This study reveals how TFIID, a transcription factor, interacts with E-protein activation domain 3 (AD3) via TAF4. This interaction enhances TFIID binding to promoters, facilitating gene activation.

Keywords:
E proteinsE2AHEBTAF4TAFHTFIIDtranscription coactivator

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Related Experiment Videos

Last Updated: May 9, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
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Published on: May 1, 2020

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Published on: February 25, 2012

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
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Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Protein-Protein Interactions

Background:

  • The transcription factor II D (TFIID) complex is essential for initiating gene transcription.
  • TFIID's coactivator role through interactions with activators is not well understood.

Purpose of the Study:

  • To elucidate the mechanism by which TFIID functions as a coactivator.
  • To investigate the direct interaction between TFIID and E-proteins.

Main Methods:

  • Biochemical assays to study TFIID-E-protein interactions.
  • In vivo assays to assess gene activation and TFIID recruitment.

Main Results:

  • A direct interaction between TFIID and an E-protein activation domain (AD3) was identified, mediated by TAF4's TAF homology (TAFH) domain.
  • This interaction is crucial for E-protein-mediated activation of a natural target gene.
  • The interaction enhances TFIID binding to core promoter elements, facilitating transcription initiation.
  • The TAFH domain plays a gene-specific role in TFIID recruitment and activation in vivo.

Conclusions:

  • TAF4 acts as a coactivator for E-proteins.
  • Facilitated TFIID binding, driven by direct E-protein interaction, is a key mechanism for gene activation.