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Crystallization and preliminary x-ray diffraction study of neurotoxin-I from Naja naja oxiana venom
A M Mikhailov1, A V Nickitenko, S D Trakhanov
1Institute of Crystallography of the USSR Academy of Sciences, Moscow.
FEBS Letters
|August 20, 1990
Abstract:
Crystals of the neurotoxin-I (NTX-I) from the venom of the middle Asian cobra Naja naja oxiana have been grown by vapour diffusion and dialysis methods. The crystals belong to space group P2(1)2(1)2 with dimension of a = 25.19 A, b = 75.59 A, c = 36.09 A and diffract to 1.9 A resolution. The asymmetric unit contains one molecule (Vm = 2.2 A/Da). Using the molecule of alpha-cobratoxin (CTX) as a starting model for NTX-I structure determination coordinates of C alpha atoms of the NTX-I molecule were obtained and the position of NTX-I in the unit cell was derived.