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The influence of oligonucleotide-effector on the selectivity of sequence specific modification of 16 S rRNA
M A Zenkova1, O S Fedorova, A S Levina
1Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of USSR Academy of Sciences.
FEBS Letters
|August 20, 1990
Abstract:
The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide-effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 microM effector at 5 microM concentration of the reagent.