Related Experiment Video
Updated: May 9, 2026
Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection
Published on: March 14, 2019
In vitro elution characteristics of vancomycin in a composite calcium phosphate/calcium sulfate bone substitute
Xu Yang1, Liza Osagie, Mathias P Bostrom
1Hospital for Special Surgery, 535 East 70th St., New York, NY 10021 USA.
Background:
Periprosthetic joint infection is a particularly difficult orthopedic problem, complicating a growing number of revision procedures. Joint debridement and systemic antibiotics are the mainstay of treatment, yet difficulty remains in maintaining a minimum inhibitory concentration of antibiotic at the localized site of infection.
Study Aims:
This study analyzes the elution characteristics of a 40%bwt calcium phosphate-60%bwt calcium sulfate composite, at varying concentrations of vancomycin.
Methods:
Four groups of varying concentrations of vancomycin (2.63%bwt, 5.13%, 9.76%, and 17.78%) were mixed with one pack of the composite cement. At designated time intervals up to 28 days, the antibiotic concentration was detected using fluorescence polarization immunoassay and the elution trends compared.
Results:
The elution rate of each of the four groups decreased over time. At almost all of the intervals, the elution rates of the higher concentration groups were significantly higher than the lower concentration groups (P < 0.05).
Conclusions:
Calcium sulfate reabsorbs over a prolonged period, producing porosity which allows for new bone ingrowth through occupation of osteoprogenitor cells and osteoblasts; while calcium phosphate acts as a long-term osteoconductive matrix.
Clinical Relevance:
The results of this study suggest that vancomycin can be mixed affectively with a calcium sulfate/phosphate composite, both maintaining stability and eluting gradually over a clinically relevant period of time.
