Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and characterization of an endogenous pararetrovirus in Gentiana triflora plants.

Archives of virology·2026
Same author

A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa.

The New phytologist·2026
Same author

Fine-tuning quantitative agronomic traits by manipulating gene copy number in rice.

The New phytologist·2026
Same author

Multi-omics dissection of steviol glycoside synthesis reveals haplotype-linked specialization of UGT76G genes in Stevia rebaudiana.

The New phytologist·2026
Same author

Impact of nighttime centralization on timeliness of care for pediatric acute scrotum: A regional retrospective study in Yamanashi prefecture, Japan.

Journal of pediatric urology·2026
Same author

Drivers and Consequences of Size Declines in Unicells.

Ecology letters·2026

Related Experiment Video

Updated: May 9, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

MutMap+: genetic mapping and mutant identification without crossing in rice.

Rym Fekih1, Hiroki Takagi, Muluneh Tamiru

  • 1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Plos One
|July 23, 2013
PubMed
Summary

MutMap+ is a new method for identifying causal mutations in plants. It uses whole genome sequencing of M3 progeny to find genetic variations without needing artificial crosses, making it useful for difficult-to-breed crops.

More Related Videos

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
12:42

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Related Experiment Videos

Last Updated: May 9, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
12:42

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

Published on: April 13, 2012

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
09:32

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Area of Science:

  • Genomics
  • Plant Genetics
  • Molecular Biology

Background:

  • Advances in genome sequencing enable rapid association of phenotypic variation with genomic differences.
  • The MutMap method was developed for rapid identification of causal nucleotide changes in rice mutants using whole genome resequencing.
  • MutMap relies on F2 progeny from crosses between mutants and the parental line.

Purpose of the Study:

  • To describe MutMap+, a versatile extension of the MutMap method.
  • To enable identification of causal mutations without artificial crossing.
  • To facilitate gene isolation in crops that are recalcitrant to artificial crosses.

Main Methods:

  • MutMap+ identifies causal mutations by comparing SNP frequencies in bulked DNA.
  • It utilizes M3 generation progeny derived from selfing of M2 heterozygous individuals.
  • Whole genome resequencing of pooled DNA from mutant and wild-type progeny is employed.

Main Results:

  • MutMap+ successfully identifies causal mutations.
  • The method does not necessitate artificial crossing between mutants and wild-type lines.
  • It is suitable for mutations causing early lethality, sterility, or crossing difficulties.

Conclusions:

  • MutMap+ is a versatile and efficient tool for identifying causal mutations.
  • This method expands the applicability of MutMap to a wider range of genetic studies and crop species.
  • MutMap+ is particularly valuable for gene isolation in crops with challenging breeding systems.