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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

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Updated: May 9, 2026

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Published on: February 10, 2023

Heritable and precise zebrafish genome editing using a CRISPR-Cas system.

Woong Y Hwang¹, Yanfang Fu, Deepak Reyon

¹Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America.

|July 23, 2013

Summary

This summary is machine-generated.

CRISPR RNA-guided Cas9 nucleases efficiently create heritable mutations in zebrafish. This system enables precise genome editing, including single nucleotide substitutions, and expands targeting range for advanced genetic studies.

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Last Updated: May 9, 2026

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Published on: February 10, 2023

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Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

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Published on: August 28, 2018

Area of Science:

Genetics
Molecular Biology
Zebrafish Model Organisms

Background:

CRISPR RNA-guided Cas9 nucleases (RGNs) are established tools for somatic indel mutations in zebrafish.
Previous RGN systems were limited to somatic gene modification.

Purpose of the Study:

To demonstrate the heritability of RGN-induced mutations in zebrafish.
To enhance RGN capabilities for precise genome editing using single-stranded oligodeoxynucleotides (ssODNs).
To improve the targeting efficiency of RGNs.

Main Methods:

Utilized CRISPR RNA-guided Cas9 nuclease system in zebrafish.
Employed single-stranded oligodeoxynucleotides (ssODNs) for precise sequence modifications.
Developed strategies to increase RGN targeting range.

Main Results:

Achieved 100% germline transmission efficiency for RGN-induced mutations.
Successfully created precise sequence modifications, including single nucleotide substitutions, using ssODNs.
Improved RGN targeting range from 1 in 128 bp to 1 in 8 bp.

Conclusions:

RGN-induced mutations are heritable in zebrafish, significantly expanding their utility.
The RGN system, combined with ssODNs, allows for precise and heritable genome modifications.
Enhanced targeting strategies broaden the application of CRISPR-Cas in zebrafish for genetic research.