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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Visible-light Induced Reduction of Graphene Oxide Using Plasmonic Nanoparticle
07:24

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Published on: September 22, 2015

Multicolour probes for sequence-specific DNA detection based on graphene oxide.

Qing Zhu1, Dongshan Xiang, Cuiling Zhang

  • 1Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, P. R. China.

The Analyst
|July 23, 2013
PubMed
Summary

Graphene oxide enables sensitive detection of multiple viruses by adsorbing DNA and quenching dye signals. This bifunctional material offers a novel approach for multiplexed viral DNA detection.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Nanotechnology

Background:

  • Graphene oxide (GO) possesses unique bifunctional properties.
  • GO can adsorb single-stranded DNA (ssDNA) and quench organic dye fluorescence.

Purpose of the Study:

  • To develop a multiplexed DNA detection system using GO's bifunctionality.
  • To achieve sensitive and selective detection of specific viral DNA sequences.

Main Methods:

  • Utilizing GO's adsorption of ssDNA.
  • Leveraging GO's fluorescence quenching capability.
  • Developing a multiplexed assay for simultaneous detection.

Main Results:

  • Demonstrated sensitive detection of specific viral DNA targets.
  • Achieved selective identification of Human Immunodeficiency Virus (HIV), Vexillovirus (VV), and Enterovirus (EV).
  • Validated the efficacy of the GO-based multiplexed detection system.

Conclusions:

  • Graphene oxide serves as an effective platform for multiplexed DNA detection.
  • The developed system offers a sensitive and selective method for identifying multiple viral pathogens.
  • This approach holds promise for advanced diagnostic applications.