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A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

Published on: December 18, 2015

Murine multicolor banding.

N Kosyakova¹, V Trifonov, S Romanenko

¹Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Jena, Germany. Nadezda.Kosyakova@mti-jena.de

|July 24, 2013

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Multicolor banding (MCB) is now an optimal method for mouse chromosome karyotyping. This study presents MCB probe sets for all mouse chromosomes, enhancing their application in research.

Area of Science:

Genetics and Genomics
Cytogenetics
Molecular Biology

Background:

The multicolor banding (MCB) technique, initially developed for human karyotyping, offers high resolution for chromosome analysis.
Murine (mouse) models are crucial in biomedical research, necessitating accurate methods for chromosome analysis.

Purpose of the Study:

To establish and present multicolor banding (MCB) probe sets for all murine autosomes and the X-chromosome.
To review the potential applications of these MCB probe sets in murine karyotyping.

Main Methods:

Development and validation of specific MCB probe sets targeting unique sequences on each murine chromosome.
Application of the established MCB probe sets for high-resolution karyotyping of mouse chromosomes.

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Last Updated: May 9, 2026

A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice

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Published on: December 18, 2015

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

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Published on: March 30, 2020

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Published on: September 22, 2013

Main Results:

Successfully established MCB probe sets for all murine autosomes and the X-chromosome.
Demonstrated the efficacy of MCB for detailed murine chromosome banding and identification.

Conclusions:

The developed MCB probe sets provide an optimal and efficient approach for murine karyotyping.
These MCB probe sets significantly advance the potential applications in genetic research using mouse models.