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Tumor cells augment the factor Xa-catalyzed conversion of prothrombin to thrombin
1Department of Pathology, University of New Mexico School of Medicine, Albuquerque.
Haemostasis
|January 1, 1990
Summary
Tumor cells can activate blood coagulation by forming a prothrombinase complex, aiding metastasis. Some cancer cells synthesize factor V, enhancing thrombin generation and fibrin deposition.
Area of Science:
- Biochemistry
- Oncology
- Hematology
Background:
- Blood coagulation and fibrin deposition are implicated in tumor metastasis.
- Tumor cells' role in activating coagulation is not fully understood.
Purpose of the Study:
- To investigate the ability of human tumor cell lines to augment prothrombin activation.
- To determine if tumor cells synthesize or express components of the coagulation cascade.
Main Methods:
- Assessed thrombin formation using chromogenic substrate assays.
- Utilized anti-human factor V IgG for inhibition studies.
- Employed biosynthetic labeling and immunoprecipitation to detect factor V synthesis.
- Studied factor Va binding kinetics to tumor cells.
Main Results:
- All four tumor cell lines (COLO 205, HepG2, J82, CAPAN-2) accelerated prothrombin activation.
- COLO 205 and HepG2 cells synthesized factor V or a factor-V-like molecule.
- These cells also bound exogenous factor Va, enhancing prothrombin activation.
- J82 and CAPAN-2 cells lacked detectable factor V but could bind exogenous factor Va.
Conclusions:
- Tumor cells can assemble a functional cell surface prothrombinase complex.
- This complex formation may contribute to fibrin deposition and tumor progression.
- Tumor cell-derived factor V plays a role in prothrombin activation for specific cell lines.