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A microfluidic SPLITT device for fractionating low-molecular weight samples.

Tristan F Kinde1, Debashis Dutta

  • 1Department of Chemistry, University of Wyoming, Laramie, Wyoming 82071, United States.

Analytical Chemistry
|July 24, 2013
PubMed
Summary
This summary is machine-generated.

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This study presents a microfluidic split flow thin cell (SPLITT) fractionation device for high-resolution separation of low-molecular weight samples. A novel surface coating effectively minimizes electroosmotic flow, enabling efficient inline analysis with mass spectrometry.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Microfluidic devices offer precise control for chemical analyses.
  • Electrophoretic separations require minimizing disruptive fluid dynamics.
  • Effective fractionation of low-molecular weight samples is crucial for purity assessment.

Purpose of the Study:

  • To design and validate a microfluidic split flow thin cell (SPLITT) fractionation device for high-resolution separation.
  • To address the challenge of electroosmotic flow in microfluidic channels.
  • To enable inline, label-free detection of fractionated analytes using mass spectrometry.

Main Methods:

  • Fabrication of a SPLITT fractionation device with internal electrodes.
  • Chemical surface modification of microfluidic conduits using N-(2-triethoxysilylpropyl) formamide.

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  • Integration of the SPLITT device with a mass spectrometer via electrospray ionization.

    • Main Results:

    • Achieved lateral electric fields of 100 V/cm and separation distances of 500 μm for high-resolution fractionation.
    • Demonstrated a reduction in electroosmotic flow by over 5 orders of magnitude via surface modification.
    • Attained over 95% product purity for peptide samples using the developed SPLITT system.

    Conclusions:

    • The developed SPLITT device enables high-resolution fractionation of low-molecular weight samples.
    • Surface modification effectively mitigates electroosmotic flow, enhancing separation efficiency.
    • Inline mass spectrometry detection allows for label-free analysis and purity assessment.