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Updated: May 9, 2026
A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood
Published on: August 15, 2025
Description of a double centrifugation tube method for concentrating canine platelets
Anna Perazzi1, Roberto Busetto, Tiziana Martinello
1Department of Animal Medicine, Production and Health, University of Padova, Padova, Italy. anna.perazzi@unipd.it
Background:
To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double centrifugation tube method to obtain platelet concentrates, which were then analyzed by a flow cytometry haematology system for haemogram. Platelet concentration and white blood cell count were determined in all samples.
Results:
Platelet concentration at least 4 times higher than the baseline value and a white blood cell count not exceeding twice the reference range were obtained respectively in 10 cases out of 12 (83.3%) and 11 cases out of 12 (91.6%).
Conclusions:
This double centrifugation tube method is a relatively simple and inexpensive method for obtaining platelet-rich canine plasma, potentially available for therapeutic use to improve the healing process.
