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Related Experiment Video

Updated: May 9, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Global and gene specific DNA methylation changes during zebrafish development.

Xiefan Fang1, Jone Corrales, Cammi Thornton

  • 1Department of Pharmacology and Environmental Toxicology Research Program, School of Pharmacy, University of Mississippi, University, MS 38677, USA.

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|July 24, 2013
PubMed
Summary

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This summary is machine-generated.

DNA methylation patterns change throughout an organism's life. In zebrafish, DNA methylation is low in early embryos but increases later, with gene expression changes not always correlating with methylation patterns.

Area of Science:

  • Epigenetics
  • Developmental Biology
  • Zebrafish Model Organism

Background:

  • DNA methylation is a crucial epigenetic mechanism that undergoes dynamic changes during an organism's lifespan.
  • Prior research has largely concentrated on DNA methylation shifts during the earliest stages of embryogenesis.

Purpose of the Study:

  • To compare global and gene-specific DNA methylation patterns in zebrafish (Danio rerio) across different life stages: embryos, larvae, and adult livers.
  • To investigate the correlation between DNA methylation patterns and gene expression of key developmental genes.

Main Methods:

  • Global DNA methylation analysis in zebrafish embryos, larvae, and adult livers.
  • Quantitative real-time PCR (qPCR) to assess mRNA expression levels of DNA methyltransferase 1 (dnmt1) and glycine N-methyltransferase (gnmt).
Keywords:
CGICpGCpG islandCytosine phosphate guanineDNA methylationDNA methyltransferaseGlycine N-methyltransferaseHCPHigh CpG promoterHours post fertilizationICPIntermediate CpG promoterLCPLow CpG promoterPGCsPrimordial germ cellsRas association domain family member 1TSSTelomerase reverse transcriptaseTranscriptional start sitedevelopmentdnmtgnmthpfrassf1tertvasazebrafish

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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

Related Experiment Videos

Last Updated: May 9, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
10:17

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

  • Analysis of DNA methylation patterns and gene expression for five specific genes: vasa, Ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun, and c-myca.

    • Main Results:

    • Zebrafish embryos showed low percent cytosine methylation between 2 to 4.3 hours post fertilization (hpf), with consistently higher methylation observed in zebrafish older than 6 hpf.
    • Early embryogenesis exhibited high DNA methyltransferase 1 (dnmt1) and low glycine N-methyltransferase (gnmt) mRNA expression.
    • The timing of DNA methylation pattern changes was found to be gene-specific for the studied developmental genes.
    • Observed changes in gene expression did not consistently correlate with the corresponding DNA methylation patterns.

    Conclusions:

    • DNA methylation is a dynamic process in zebrafish, varying significantly from early embryogenesis to later life stages.
    • Gene-specific regulation of DNA methylation occurs during development, and its relationship with gene expression is complex and not always direct.
    • This study highlights the intricate interplay between DNA methylation and gene expression during zebrafish development, emphasizing gene-specific dynamics.