Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A pH-dependent macroglobulin gel.

P W Rand, M D Lovell, E Lacombe

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |May 15, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reciprocal and multi-species chromosome BAC painting in crucifers (Brassicaceae).

    Cytogenetic and genome research·2010
    Same author

    Tick (Acari: Ixodidae) infestation at two rural, seasonal camps in Maine and Vermont.

    The Journal of parasitology·2009
    Same author

    Assessment of metabolic changes in the striatum of a rat model of parkinsonism: an in vivo (1)H MRS study.

    NMR in biomedicine·2009
    Same author

    Microcephaly is not mandatory for the diagnosis of mosaic variegated aneuploidy syndrome.

    American journal of medical genetics. Part A·2005
    Same author

    Attempt to control ticks (Acari: Ixodidae) on deer on an isolated island using ivermectin-treated corn.

    Journal of medical entomology·2004
    Same author

    Enzootic Babesia microti in Maine.

    The Journal of parasitology·2003
    Same journal

    Reference intervals for venous blood gas measurement in a healthy Chinese population.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Multiplex methylation marker analysis for ctDNA detection in liquid biopsies from anal cancer patients: an HPV-independent approach.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Development and validation of patient-based exponentially weighted moving average quality control models for three antipsychotic drugs and their metabolites by LC-MS/MS.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Comparing conventional correction formulas and machine learning-based prediction of ionized calcium.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Micro- and nanoplastics as emerging clinical analytes: analytical validation, interpretive uncertainty, and laboratory actionability in human specimens.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Performance of Free Light Chain reagents in the Dutch External Quality Assessment programme over the past 14 years.

    Clinica chimica acta; international journal of clinical chemistry·2026
    See all related articles

    Waldenstrom's macroglobulinemia plasma gels in air due to pH changes, not temperature or CO2. This pH-dependent solubility of IgM impacts its analysis and clinical behavior.

    Area of Science:

    • Biochemistry
    • Hematology
    • Protein Chemistry

    Background:

    • Waldenstrom's macroglobulinemia is characterized by the overproduction of monoclonal IgM antibodies.
    • The physical properties of plasma proteins, such as IgM, can be influenced by environmental factors.
    • Understanding these properties is crucial for accurate diagnosis and treatment monitoring.

    Purpose of the Study:

    • To investigate the cause of plasma gelation observed in a patient with Waldenstrom's macroglobulinemia.
    • To determine the relationship between pH variations and the solubility of IgM.
    • To elucidate the clinical and analytical implications of IgM solubility changes.

    Main Methods:

    • Plasma samples from a Waldenstrom's macroglobulinemia patient were analyzed under varying conditions.

    Related Experiment Videos

  • Studies focused on the effects of temperature, carbon dioxide tension, and hydrogen ion concentration (pH).
  • Viscosity and precipitation of purified IgM were measured across a range of pH values.

    • Main Results:

    • Plasma gelation was found to be primarily dependent on hydrogen ion concentration (pH) variations, not temperature or CO2.
    • Purified IgM exhibited increased viscosity between pH 7.5 and 8.0.
    • IgM precipitated between pH 8.0 and 9.7, and redissolved reversibly between pH 9.7 and 10.0.

    Conclusions:

    • The observed gelation of Waldenstrom's macroglobulinemia plasma is attributed to pH-dependent solubility of IgM.
    • These solubility characteristics of IgM have significant implications for laboratory analysis.
    • Understanding IgM's pH-dependent behavior is essential for managing patients with Waldenstrom's macroglobulinemia.