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Pyrosequencing: A Simple Method for Accurate Genotyping
Published on: January 8, 2008
Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach
1State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China.
Journal of Microbiological Methods
|July 24, 2013
Abstract:
We described a triplex polymerase chain reaction (PCR) and triplex pyrosequencing assay which allowed a simultaneous determination of three tag single nuleotide polymorphisms (tag SNPs) in the lipopolysaccharide-binding protein (LBP) gene: rs1780623, rs11536972 and rs2232618. This method enables a fast and cost-effective genotyping and a simultaneous determination of the three tag SNPs.

