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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for electronic transitions. As a result...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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Related Experiment Video

Updated: May 9, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

High-throughput, high-resolution Echelle deep-UV Raman spectrometer.

Sergei V Bykov1, Bhavya Sharma, Sanford A Asher

  • 1Department of Chemistry, University of Pittsburgh, Pittsburgh, PA 15260, USA.

Applied Spectroscopy
|July 24, 2013
PubMed
Summary
This summary is machine-generated.

We developed a novel deep ultraviolet Raman spectrograph with unprecedented throughput and resolution. This advanced instrument enables sensitive detection of subtle changes in peptide bond composition.

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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
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Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

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Last Updated: May 9, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Published on: December 30, 2025

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

  • Spectroscopy
  • Analytical Chemistry
  • Biophysics

Background:

  • Deep ultraviolet (UV) Raman spectroscopy offers unique insights into molecular vibrations.
  • Conventional deep UV Raman spectrometers often suffer from low throughput and resolution, limiting their sensitivity.

Purpose of the Study:

  • To design and construct an ultrahigh-throughput, high-resolution deep UV Raman spectrograph.
  • To demonstrate the capability of the new spectrograph for sensitive monitoring of molecular composition changes.

Main Methods:

  • The spectrograph integrates a high-efficiency filter-stage monochromator and a high-dispersion Echelle spectrograph.
  • The system comprises six mirrors and two gratings, optimized for efficiency at 229 nm.
  • Resolution was characterized using Rayleigh scattering, with a limiting resolution of 0.6 cm⁻¹ FWHM.

Main Results:

  • The spectrograph achieves an overall efficiency of ~18% at 229 nm.
  • A throughput 35-fold greater than conventional spectrometers was measured.
  • The instrument enables ultrahigh signal-to-noise ratio deep UV Raman spectra.

Conclusions:

  • The developed deep UV Raman spectrograph offers significant improvements in throughput and resolution.
  • This advancement allows for the detection of <1% changes in peptide bond composition.
  • The spectrograph is a powerful tool for sensitive molecular analysis in various scientific fields.