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Correlation between phylogroups and intracellular proteomes of Propionibacterium acnes and differences in the protein

Itaru Dekio1, Renata Culak, Min Fang

  • 1Department for Bioanalysis and Horizon Technologies, Microbiology Services, Health Protection Agency Colindale, London NW9 5EQ, UK. dekio@mx2.et.tiki.ne.jp

Biomed Research International
|July 24, 2013
PubMed

Insights

Propionibacterium acnes, a skin bacterium, shows distinct protein expression profiles across its evolutionary types. These proteomic differences correlate with genetic clades and may be influenced by the microenvironment.

Area of Science:

  • Microbiology
  • Proteomics
  • Genomics

Background:

  • Propionibacterium acnes is a common human skin commensal and opportunistic pathogen.
  • P. acnes comprises three major evolutionary clades (types I, II, and III).

Purpose of the Study:

  • To investigate protein expression differences among P. acnes phylogroups.
  • To correlate proteomic profiles with genetic clades.

Main Methods:

  • Proteomic analysis of 21 P. acnes strains using SELDI-TOF mass spectrometry, capillary gel electrophoresis, and LC-MS/MS.
  • Proteins extracted from cells cultured under anaerobic and aerobic conditions.
  • Data visualized via heat map and dendrogram.

Main Results:

  • Four distinct proteomic groups were identified, showing congruence with genetic clades.
  • Type I strains were in proteome Group A; type III strains were in Group B.
  • Groups C and D contained mixtures of types I and II, unaffected by culture conditions.
  • A type IB strain showed high expression of methylmalonyl-CoA epimerase and CAMP factor under anoxic conditions.

Conclusions:

  • Genomic and proteomic data show good congruence.
  • The microenvironment of P. acnes subtypes may influence protein expression.

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