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Updated: May 9, 2026
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
[A preliminary study on vascular endothelial growth factor C gene modified lymph node transplantation in promoting
Nanhang Lu1, Yong Zhang, Zihao Feng
1Department of Plastic & Reconstructive Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, PR China.
Objective:
To investigate the effects of vascular endothelial growth factor C (VEGF-C) gene modified lymph nodes on promoting proliferation of lymphatic endothelial cells in the surrounding tissues.
Methods:
Thirty-six Sprague Dawley rats, weighing 200.1-271.5 g, were randomly divided into 2 groups (n=18). After the in situ axillary lymph nodes transplantation models were established in both groups, 1.5 x 10(8) PFU Ad-VEGF-C-Flag and Ad-Flag were injected into the transplanted lymph nodes in experimental group and control group, respectively. At 3 days after injection, the axillary lymph nodes were harvested to observe the expression of Flag; at 1, 2, and 4 weeks after injection, the axillary lymph nodes and the surrounding tissues were harvested to observe the expression of Prxo-1 protein and to calculate the fluorescence density; at 2 and 4 weeks after injection, the absorbance (A) value of treated blood at 620 nm was calculated to observe lymphatic backflow function improvement; the rats without treatment served as normal control group, and the rats with in situ axillary lymph
Results:
At 3 days after injection, the expression of Flag could be nodes transplantation model served as blank control group. detected in experimental group and control group. The fluorescence density of Prox-1 protein in experimental group increased at 1, 2, and 4 weeks, and it was significantly higher than that in control group (P < 0.05). The A values of normal control group and blank control group were 0.539 +/-0.020 and 0.151 +/- 0.007, respectively. The A values of experimental group and control group were 0.170 +/- 0.011 and 0.168 +/- 0.010 at 2 weeks, and 0.212 +/- 0.016 and 0.197 +/- 0.006 at 4 weeks, which were significantly lower than those of normal control group (P < 0.05), but no significant difference was found when compared with blank control group, and between the experimental group and control group (P > 0.05).
Conclusion:
The VEGF-C gene modified lymph nodes can stimulate the proliferation of lymphatic endothelial cells in the surrounding tissues. However, it has no improved effect on lymphatic back-flovi function in the affected limb.
