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Aptamer-based label-free detection of PDGF using ruthenium(II) complex as luminescent probe

E Babu1, S Singaravadivel, P Manojkumar

  • 1Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625 021, India.

Analytical and Bioanalytical Chemistry
|July 25, 2013
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...