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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Triterpenoids and steroids with cytotoxic activity from Emmenopterys henryi
Xing-De Wu1, Juan He, Xing-Yao Li
1State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, P. R. China.
Abstract:
Two new ursane-type triterpenoids, 3β,19α,23-trihydroxyurs-12-en-24-al-28-oic acid (1) and 3β,19α,24-trihydroxy-23-norurs-12-en-28-oic acid (2), two new pregnane derivatives, 3β,12β-dihydroxy-5α-pregnane-14,16-dien-20-one (9) and 12β-hydroxy-5α-pregnane-14,16-dien-3,20-dione (10), and eight known compounds were isolated from the twigs and leaves of Emmenopterys henryi. The structures of the new compounds were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR experiments. Compounds 4, 11, and 12 showed cytotoxicity against HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cell lines with IC50 values in the range of 3.11-20.12 µM.
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