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Updated: Oct 13, 2026

The Dimethylnitrosamine Induced Liver Fibrosis Model in the Rat
Published on: June 17, 2016
[Production of cyanide in the rat liver from 3-aminopropionitrile]
G M Fernandez1, J M Lumbreras, R Balaña
1Dpto. de Farmacología y Toxicología, Facultad de Veterinaria, Universitad Complutense, Madrid, España.
Abstract:
CN- Production was investigated from a saturated mononitrile, 3-aminopropionitrile, in small liver pieces and liver homogenates. Results show that CN- production is a function of time and of the concentration of active ingredient, with a trend to saturation in both cases. Similarly, cell integrity is not required for the biotransformation of 3-aminopropionitrile to occur. Cofactors such as NADPH must be present to reach high level activities. The effect of free radicals scavengers such as mannitol and DMSO was also studied.
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