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Related Experiment Video

Updated: May 9, 2026

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Amine-reactive neutron-encoded labels for highly plexed proteomic quantitation.

Alexander S Hebert1, Anna E Merrill, Jonathan A Stefely

  • 1Department of Biomolecular Chemistry, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706;

Molecular & Cellular Proteomics : MCP
|July 25, 2013
PubMed
Summary

Researchers developed neutron-encoded (NeuCode) chemical labels for high-plex protein quantification using mass spectrometry. This novel method offers comparable performance to existing strategies, enabling detailed proteomic analysis.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Accurate and high-plex protein quantification is crucial for understanding biological processes.
  • Existing methods like metabolic labeling and isobaric tagging have limitations.
  • Novel chemical labeling strategies are needed to improve quantitative proteomics.

Purpose of the Study:

  • To introduce a novel amine-reactive chemical label, neutron-encoded (NeuCode) tags, for mass spectrometry (MS)-based protein quantification.
  • To demonstrate the capability of NeuCode tags for up to 12-plex quantitative proteomics.
  • To evaluate the quantitative performance and spectral characteristics of the NeuCode labeling strategy.

Main Methods:

  • Development of amine-reactive chemical labels exploiting differential neutron-binding energy between Carbon-13 (13C) and Nitrogen-15 (15N) isotopes.
  • Encoding of isotopic uniqueness with a 12.6-mDa mass difference between adjacent tags.
  • MS1-based protein quantification using very high mass-resolving power instruments.
  • Application of NeuCode tags for Saccharomyces cerevisiae proteome characterization during the diauxic shift.

Main Results:

  • NeuCode chemical labels enable up to 12-plex MS1-based protein quantification.
  • The mass difference between NeuCode tags does not increase spectral complexity.
  • Quantitative performance is comparable to metabolic labeling and isobaric tagging.
  • Successful characterization of Saccharomyces cerevisiae proteome during metabolic transition.

Conclusions:

  • NeuCode chemical labels offer a powerful new tool for high-plex quantitative proteomics.
  • This method combines the advantages of metabolic labeling and isobaric tagging.
  • NeuCode technology facilitates in-depth proteomic investigations, such as metabolic shifts in yeast.