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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Building nanoSPR biosensor systems based on gold magnetic composite nanoparticles.
Dong Yang1, Jianzhong Ma, Mingli Peng
1College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Journal of Nanoscience and Nanotechnology
|July 26, 2013
Summary
We developed novel Fe3O4/Au nanoparticle probes for biomolecular detection. The MUA-modified probes showed higher sensitivity and faster reaction rates than PAA-modified probes in colorimetric assays.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Composite Fe3O4/Au nanoparticles offer unique properties for biomolecular detection.
- Localized surface plasmon resonance (LSPR) biosensors are valuable tools in diagnostics.
Purpose of the Study:
- To fabricate and compare two nanoSPR biosensors using Fe3O4/Au nanoparticles modified with MUA and PAA.
- To evaluate the sensitivity and stability of these biosensors for colorimetric detection of goat anti-rabbit IgG.
Main Methods:
- Fabrication of Fe3O4/Au nanoparticles modified with 11-mercaptoundecanoic acid (MUA) and poly acrylic acid (PAA).
- Conjugation of rabbit IgG to the modified nanoparticles.
- Colorimetric detection assay using goat anti-rabbit IgG as the target analyte.
- Comparative analysis of MUA- and PAA-modified biosensor performance, including sensitivity and kinetic studies.
Main Results:
- PAA-modified nanoparticles exhibited better dispersion and ionic independent stability, suitable for colorimetric detection.
- MUA-modified nanoparticle probes demonstrated higher sensitivity (50 nmol/L) in SPR detection.
- Kinetic studies revealed a second-order reaction, with MUA-probes showing twice the reaction rate of PAA-probes.
Conclusions:
- The developed nanoSPR biosensor fabrication protocol is effective for creating molecular probes.
- Composite Fe3O4/Au nanoparticles show promise as ideal probes for sensitive, selective, and real-time biomolecular detection.
