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Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...

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Related Experiment Video

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Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
05:46

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Published on: April 9, 2014

Counselor-based rapid HIV testing in community pharmacies.

Yvette Calderon1, Ethan Cowan, John Y Rhee

  • 1Department of Emergency Medicine, Jacobi Medical Center, Bronx, NY 10461, USA. ycalderon@gmail.com

AIDS Patient Care and Stds
|July 26, 2013
PubMed
Summary

Community pharmacies offer effective rapid HIV testing, with a 0.3% incidence rate. This counselor-based program in New York City pharmacies demonstrated participant satisfaction and successful HIV screening.

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Area of Science:

  • Public Health
  • Epidemiology
  • Clinical Pharmacy

Background:

  • Community pharmacies are increasingly recognized as accessible venues for public health services.
  • Implementing rapid HIV testing in pharmacies can improve early detection and linkage to care.
  • Previous HIV testing rates among the target population were high, indicating a need for accessible re-testing options.

Purpose of the Study:

  • To evaluate the implementation and outcomes of a counselor-based rapid HIV testing program within community pharmacies.
  • To assess the feasibility and client satisfaction of pharmacy-based HIV screening.
  • To determine the HIV incidence and characteristics of newly diagnosed individuals in this setting.

Main Methods:

  • A prospective cross-sectional study was conducted in five community pharmacies in New York City.
  • A convenience sample of 2030 individuals who agreed to testing from 2805 eligible clients participated.
  • Data collected included demographics, previous testing history, insurance status, HIV test results, and CD4 cell counts.

Main Results:

  • The HIV incidence rate was 0.3% among tested individuals.
  • The median CD4 cell count for newly diagnosed individuals was 622.0.
  • Participants reported high satisfaction with the counselor-based rapid HIV testing program.
  • A significant portion of participants (34%) were uninsured, highlighting the role of pharmacies in reaching vulnerable populations.

Conclusions:

  • Counselor-based rapid HIV testing programs are feasible and well-received in community pharmacy settings.
  • Community pharmacies can serve as effective sites for HIV screening, contributing to early diagnosis and public health.
  • The program successfully identified new HIV cases and demonstrated high client satisfaction, supporting its expansion.