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Related Experiment Video

Updated: May 9, 2026

Production and Analysis of Sporosarcina pasteurii Biocement Bricks Using Custom 3D-Printed Molds for Unconfined Compression Tests

Production and Analysis of Sporosarcina pasteurii Biocement Bricks Using Custom 3D-Printed Molds for Unconfined Compression Tests

Published on: March 7, 2025

Biocalcification using B. pasteurii for strengthening brick masonry civil engineering structures.

Supriya H Raut¹, D D Sarode, S S Lele

¹Department of Food Engineering and Technology, Institute of Chemical Technology, N. P. Marg, Matunga, Mumbai, 400019, India.

World Journal of Microbiology & Biotechnology

|July 26, 2013

Summary

Bacillus pasteurii bacteria enhanced brick strength by 83.9% through microbiologically induced calcite precipitation in optimized media. This biocalcification also reduced water absorption by 48.9%, improving brick durability for construction applications.

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Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii

Published on: April 16, 2016

Area of Science:

Biotechnology
Materials Science
Civil Engineering

Background:

Construction materials like bricks are susceptible to degradation.
Microbiologically induced calcite precipitation (MICP) offers a novel approach to material enhancement.
Bacillus pasteurii is known for its urease activity, crucial for MICP.

Purpose of the Study:

To investigate the efficacy of Bacillus pasteurii for calcite precipitation in bricks.
To evaluate the impact of optimized urease production media (OptU) on biocalcification.
To assess the effect of MICP on brick compressive strength and water absorption.

Main Methods:

Culturing Bacillus pasteurii (NCIM 2477) in optimized urease production media (OptU) and standard nutrient broth (NB).
Treating bricks with bacterial cultures and monitoring parameters (pH, growth, urease activity, urea breakdown, calcite precipitation) over 28 days.
Measuring compressive strength and water absorption capacity of treated and control bricks.

Main Results:

Bricks treated with B. pasteurii in OptU media showed an 83.9% increase in compressive strength, compared to 24.9% with NB media.
Water absorption capacity was reduced by 48.9% in bricks treated with B. pasteurii in OptU media.
Significant calcite precipitation was observed, correlating with enhanced brick properties.

Conclusions:

Optimized media significantly enhances Bacillus pasteurii's ability to induce calcite precipitation for brick improvement.
MICP using B. pasteurii is a viable method to substantially increase brick compressive strength and reduce water absorption.
This microbial approach holds potential for commercial application in improving the lifespan of brick structures, especially heritage buildings.