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Updated: May 9, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
Published on: March 21, 2018
Cysteamine-palladium complex ([Pd(μ-OAc)(ppy)]2, ppy:2-phenylpyridine, PhMe)-modified peroxidase biosensor
Erol Akyilmaz1, M Emin Günay, Engin Asav
1Faculty of Science, Department of Biochemistry, Ege University Bornova , Izmir , Turkey.
Abstract:
A new peroxidase biosensor was developed using cysteamine-palladium complex-modified gold electrode. The principle of the measurements is based on monitoring increase in the oxidation potential of palladium complex (at + 0.47 V vs Ag/AgCl) using amperometric detection. In the optimization studies of the biosensor, effects of enzyme amount, palladium complex amount, and duration of SAM formation on biosensor responses were investigated to optimize the bioactive layer. The biosensor has a fast response time of less than 10 s to hydrogen peroxide (H2O2), with a linear range of 5.0 × 10(- 6) to 150 × 10(- 6) M and a detection limit of 3.38 × 10(- 6) M.
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