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Quantitative competitive (qc) RT-PCR as a tool in biomarker analysis

C W Evans1, D A Wilson, G N Mills

  • 1Molecular Genetics and Development, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.

Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
|July 27, 2013
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...