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A Drosophila ribosomal protein functions in mammalian cells
C G Maki1, D D Rhoads, J J Diaz
1Division of Biology, Kansas State University, Manhattan 66506.
Molecular and Cellular Biology
|September 1, 1990
Abstract:
A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.