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Generation of Recombinant Influenza Virus from Plasmid DNA
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Generation of Recombinant Influenza Virus from Plasmid DNA

Published on: August 3, 2010

Improved dual promotor-driven reverse genetics system for influenza viruses

Ahmed Mostafa1, Pumaree Kanrai, John Ziebuhr

  • 1Institute of Medical Virology, Justus Liebig University Giessen, BFS, Schubertstrasse 81, 35392 Giessen, Germany; Virology Laboratory, Environmental Research Division, National Research Center, 12311 Dokki, Giza, Egypt.

Journal of Virological Methods
|July 27, 2013
PubMed
Summary

Researchers developed a new bi-directional vector, pMPccdB, to improve reverse genetics systems for influenza A virus (IAV). This system facilitates the generation of genetically engineered IAV, including pandemic strains.

Keywords:
BGHBovine growth hormoneCEFChicken embryo fibroblastCloning vectorFFUFocus-forming unitsInfluenza virusPandemicPdmReverse genetic systemsrecombinant generatedrg

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